Friday, 25 May 2012

Start a Natural Colour Making Business

Start a Natural Colour Making Business
(320). Start a Natural Colour Making Business
Natural colors and flavors are type of food additives that are added to food and beverages to make products more appealing and tastier. The use of natural colors and flavors in food and beverage industry is increasing since last decade. Rising demand for natural foods and consumer avoidance has led to strong progress for natural colors and flavors market capturing around 30% global color and flavors market. Hyperactivity and behavioral problems in children’s due to artificial colors and flavors are other driving factors for this market. Colors and flavors derived for natural products are having Exempt from Certification. Color and flavor degradation with change in pH, light, temperature, and oxidation with other ingredient is major restrain for global natural color and flavor market.

The natural food color industry market is growing at 10%  ‐15% annually. The rationale for
growth is increasing awareness among the developed countries like USA, UK, Germany,
Europe, Japan etc. about the harmful effects and consequences of using synthetic color.
Since the product is expensive, it is consumed in countries having high income strata.
The reason for accelerating demand of the natural food colors in international market is the
growing awareness of environmental hazards of synthetic colors and harmful impact of
chemicals used for manufacturing them. European countries have not only put total ban on
manufacturing of synthetic dye based colors and the products containing such colors but
also banned the imports of products from the countries using such colors.

The global natural color and flavor market is values to be $ 1 billion in 2011 and tremendous growth potential is seen in this industry. Food is largest application segment with over 30% market share, followed by soft drinks and alcoholic beverages. Europe is the largest market for natural colors and flavors, which controls around 35% market share, followed by North America and Asia Pacific.
The report provides full analysis of the world’s leading players in the natural colors and flavors industry, the key ingredients occupying the major shares, with a clear insight and commentary on the developments and trends. With the huge market potential and growth, market is likely to witness a shift in the colors and flavors market. The market is anticipated to continue flourishing in the developed as well as the developing regions. The growth is also attributed to the growing demands and penetration of the organic and natural products

Food industry is a major segment attracting investors all over. Natural color market
products promise a good and vast market for dyes. Due to foreseen pollution problems andPage 3 of 9environmental erosions, synthetic dyes tend to be soon out of use at least in food preparations which will further give thrust to products like Annatto dyes.

Natural colours can be used in the following ways:
  • Food Industry – Frozen fish, meat, etc.
  • Beverage Industry – Soft drinks, fruit drinks, energy drinks, etc.
  • Alcoholic Beverages – Products with low pH requiring red or orange tones
  • Dairy Industry – Yogurts, ice cream and dairy based beverages
  • Confections – Candy, fillings, syrups, chewing gum, etc.
  • Fruit Preparations – Canned fruits such as cherries, Jams, Pulp, etc.
  • Cosmetic Industry – Dispersions close to eye area, eye shadows, lipsticks, etc.
  • Others – Ketchup, powdered drinks, dehydrated soups, canned soups, etc.

Growth Drivers
• Boost in demand for natural food colors in the international market due to increasing
awareness about the harmful effects of usage of synthetic colors and the chemicals
used in manufacturing them.
• Ban in trading of synthetic color made products and its manufacturing in selected
international markets like Japan and all European countries.
• Encouragement for using Natural food colors in novel products like infant toys and
crayons, organic textile printing, handmade paper etc;

Manufacturing Process

Natural colours are produced by selective extraction using solvents. These solvents extract pigments from natural sources. For example extracts of paprika and vegetable oil are refined to give oil soluble paprika oleoresin which provides an orange-red colour. Due to the chemicals being used to extract these pigments, natural colours are considered additives. As such they are subject to E-number classification and are required to declare all chemicals being used to the respective regulatory bodies.

Colouring Foodstuffs Vs Natural Colours:

Foods obtain their colour from three main sources, natural colours, browning colours produced during cooking and processing and from food additives. Natural colours consists of natural pigments which, when refined are used as additives and hence all natural colours are assigned E numbers. Similar to the challenge faced by all additive manufacturers, the use of natural colours has been largely hindered by the E-numbering system in the recent past. Unlike other additive sectors, manufacturers of natural colours have tailored this opportunity to suit their business interest by introducing "colouring foodstuffs". Colouring foodstuffs are considered by food manufacturers to be the most apt and closest alternative to natural food colours. This is because, colouring foods stuffs are extracted from natural sources (like natural colours) but are considered as ingredients and hence are not assigned E numbers (unlike natural colours).
Colouring foodstuffs are gaining popularity because they are the healthiest way to colour foods, as these colours are exclusively produced from fruits, vegetables and other edible extracts from plants. Figure 1-1 shows the production process of colouring foodstuffs. Products using colouring foodstuffs are given a clean label, as they are ingredients rather than additives. The market for colouring foodstuffs while being a comparatively new one is fast overtaking that of its more infamous counterpart, synthetic additives. The European market in particular has seen an insurgence of these products as the demand for naturally coloured food is burgeoning. Over the last few years especially there has been a noticeable shift from synthetic and natural colouring additives to colouring foodstuffs. Some of the key industry players for this sector are GNT’s, Chr Hansen’s, Overseal and Sensient Food Colours Europe. Indeed the market for these products is slated to show an increase annually by about 10 to 15 % by 2008. Fig1-1 The production of a colouring foodstuff
Market PotentialDue to a growing environmental, health and social awareness of European buyers and consumers, in many Western countries the demand for sustainable materials, products and services is increasing. As a result the market for natural dyes is also growing.

Natural Colours

Natural Colours are a healthy substitute to artificial colours, and one of our specialties is to provide advice, technical implementation assistance and supply natural colours for food products.
Suitable applications include food, drinks, pharmaceuticals and cosmetics.
Natural colours will not flavour the food in any way, merely act as pigment to colour the food in the way you desire. However there are more technical barriers to overcome with natural colours which we will be happy to advise you on.
  • Annatto (Yellow/orange)
  • Anthocyanin (Red/purple)    
  • Beetroot (Pink/blue/red)            
  • Beta Carotene (Yellow/orange)   
  • Capsanthin (Red/orange)           
  • Caramel (brown)                       
  • Carmine / Cochineal (red)          
  • Carminic Acid (orange/red)         
  • Carotene (orange)                    
  • Chlorophyll (green)
  • Chlorophyllin (green)
  • Curcumin (yellow)
  • Iron Oxide (black, red, yellow)
  • Lutein (Yellow)
  • Lycopene (Reddish Orange)
  • Riboflavoin (Yellow)
  • Titanium Dioxide (white)
  • Vegetable Carbon Black (black)

 There are two methods for extracting natural colors. One is the conventional method that is
batch type percolation which uses suitable organic solvent for extraction of the coloring
material from natural materials.
 While Super Critical Extraction (SCE) is the advance technology used for extracting natural
colors in the purest form. In India the technology for SCE has been developed by Chemical
Engineering Department IIT- Mumbai. Imported technology can be sourced from Germany
and Austria.Project Profile on Mott MacDonald
Natural Food Colors – Marigold, Annatto iNDEXTb
AF-16 3
 It can separate components in single extraction process with use of process variables like
temperature and pressure and can process multiple commodities and even improve
economic viability.
Cold Percolation
This is a traditional method of extraction used by herbalists throughout the world and it's very
simple. Above a flask or vessel is suspended a cone or tube. The bottom of the tube has a
perforated base which holds ground herb in place. Solvent is poured into the top of the tube where it soaks through the herb leaching out the extract and then falling out the bottom end of the tube into the flask. If desired, the percolation tube can be wrapped in heating tape to help facilitate the extraction.
High Pressure- Supercritical/ Sub critical Extraction
This is the most technologically advanced extraction system in the world. Super Critical Fluid
Extraction (SFE) involves taking gases, usually CO2, and compressing them into a dense liquid.This liquid is then pumped through a cylinder containing the material to be extracted. From there,the extract laden liquid is pumped into a separation chamber where the extract is separated from the gas and the gas is recovered for re-use. CO2's solvent properties can be manipulated and adjusted by varying the pressure and temperature that one works at.
The advantages of SFE are the versatility it offers in pinpointing the constituents you want to extract from a given material and the fact that your end product has virtually no solvent residues left in it. (CO2 evaporates completely) The downside is that this technology is quite expensive.There are many other gases and liquids that are highly efficient as extraction solvents when put under pressure.
Raw materials
Natural colors manufacturing, has emerged as a new opportunity for the coming period. Recently cultivation of Annatto seed has started in several parts of Gujarat that is basically done through contract farming. The adjoining states of Rajasthan (mari-gold flowers), Madhya Pradesh (Annatto seeds) and Maharashtra would also help in getting the raw material in required quantum.Gujarat’s marigold production is shown an escalating trend since 2002-03 to 2005-06 growing at an average CAGR of 54% for the last 4 years. The following table summarizes Gujarat’s marigold area under cultivation and production
Agencies to be contacted
Industrial Extension Bureau
Mott MacDonald India
Gujarat Agro Industries Corporation Ltd. 

Thursday, 24 May 2012

Start a Fish Spa Therapy Business


(319). Start a Fish Spa  Therapy Business
Dr. Fish / Garra Rufa Spa Therapy is a natural form of exfoliation and relaxation treatment that has been offered with amazing results. Doctor Fish™ have been known for softening and cleansing the skin of people who bathed in hot springs where the fish darted about.It sounds crazy, but don’t knock it until you try it – it is surprisingly fun. These normally vegetarian fish home in on areas of dry skin, especially around heels and soles of the feet and finger nails. For a psoriasis sufferer, the fish target the plaques — areas of sore, red and thickened skin.
Doctor Fish trend is made popular in Asia and has since been making the headlines in the lifestyle industry. The secret to natural beautiful skin is finally here!These Doctor Fishes (Garra Rufa) are put to work by nibbling on your skin removing dead skin cells through natural exfoliation! They have found that these tiny fishes secrete a ENZYME called diathanol which is said to improve skin regeneration!Now Imagine this, no more costly creams or products to obtain that smooth soft skin you've been wanting!The Doctor Fish Spa is a treatment which helps in the regeneration of the human skin through a natural and fun way using the Original Doctor Fish known as Garra Rufa. Most common concepts of this Doctor Fish treatment are done on the Hands & Feet also known as Fish Manicure & Fish Pedicure.
No they don’t and can’t bite, they don’t have any teeth. They suck, scrape and nibble the dead skin off your body, hands or feet.
Doctor Fish spas and skin treatment facilities are becoming more and more popular in countries like Japan, China, Turkey and across Europe. They use an amazing species of fish known as Garra rufa as a cosmetic beautifying treatment and as a cure for a variety of skin diseases. Garra rufa can be found in river basins around the Northern and Central Middle East, in countries like Turkey, Syria, Iraq and Iran, but it is now artificially bred in outdoor pools specifically for the spas.
 garrarufa1  Doctor Fish Spa
As you read on, you will be uncovering more facts about Doctor Fish & the history of it's existence. Due to the popular demand of this treatment, we have gone to the extend to secure the Doctor Fishes to bring them to you! If you are looking for home setup or business setup, it must tell you its a very profitable business.


Doctor fish is the name given to two species of fish: Garra rufa and Cyprinion Macrostomus. Other nicknames include nibble fish, kangal fish,little dermatologists and doctorfishen; in non-medical contexts, Garra Rufa is called the reddish log sucker. They live and breed in the outdoor pools of some Turkish spas, where they feed on the skin of patients with psoriasis. The fish are like combfishes in that they only consume the affected and dead areas of the skin, leaving the healthy skin to grow, with the outdoor location of the treatment bringing beneficial effects. The spas are not meant as a treatment option, only as a temporary cure for symptoms, and patients usually revisit the spas every few months. Some patients have experienced complete cure of psoriasis after repeated treatments, but due to the unpredictable nature of the disease, which is strongly influenced by endogenous factors, this may simply be regression towards the mean.

Garra Rufa occurs in the river basins of the Northern and Central Middle East, mainly in TurkeySyriaIraq and Iran. It is legally protected from commercial exploitation in Turkey due to concerns of overharvesting for export. Garra Rufa can be kept in an aquarium at home; while not strictly a "beginner's fish", it is quite hardy. For treatment of skin diseases, aquarium specimens are not well suited as the skin-feeding behavior fully manifests only under conditions where the food supply is somewhat scarce and unpredictable. In 2006, doctor fish spa resorts opened in Hakone, Japan, and in Umag, Croatia, where the fish are used to clean the bathers at the spa. There are also spas in resorts in China, such as Hainan, Belgium, The Netherlands, South Korea, Singapore, Slovakia, Surat(INDIA) and Malaysia. In 2008, the first widely known doctor fish pedicure service was opened in the United States by John Ho in Alexandria, Virginia and later in Woodbridge, Virginia, and has trademarked the treatment name of 'Dr. Fish'. The first Skin Therapy clinic using Garra Rufafish in Ireland (Limerick) was opened in 2008. These little dermatologists are used there to give relief to people with various Skin Disorders, including Eczema, Psoriasis and Dermatitis.

The so-called Doctor Fish spas first appeared in Turkey, where people suffering from a terrible skin disease called psoriasis, from all around the world started coming for treatment. Psoriasis sufferers develop raised red patches on their body covered by grey, scaly skin, which is sore or very itchy. Fortunately the Garra rufa love to nibble on dead and diseased skin and as soon as the patients insert their limbs in the water, an army of these little guys start eating away the skin thats been softened by the warm water. Doctor Fish can survive in waters as hot as 43 degrees Celsius, but nutrients cant and their not fed either, so when somebody dips their feet in their pool, its a real treat.

Specialists say Doctor Fish are not a cure for psoriasis, as the disease is influenced by many endogenous factors, but there have been cases when patients have been completely cured after several treatment sessions. But, in recent years, Doctor Fish spas have opened in Japan, China, Malaysia, South Korea or Singapore as local attraction and cosmetic facilities and less as skin disease facilities. If you enjoy taking care of your skin, you know that exfoliation is a pretty strong treatment that can only be performed once a year and only by a specialist. Thats because powerful chemicals are used, but with the help of the ravenous Doctor Fish you can say goodbye to your old skin and hello to a new healthy layer, smooth as a baby

doctor_fish1  doctor_fish2 doctor_fish3

How long does it take?
For a fish pedicure to treat feet the process will take about 10 – 15 minutes for each session. The number of sessions will depend on the skin condition. lt is often good practice to follow the fish pedicure with a massage to help further treat the skin.  Does it Hurt?No, it doesn’t hurt. You will feel a tingling sensation which many people find soothing and relaxing. This can be a little ticklish at first but you get used to it very quickly. This tickling sensation will also help promote better blood circulation.
What are the Benefits?
Doctor Fish are a fantastic way of looking after your skin. The most visible benefits are seen on people who have dry, rough or damaged skin. The fish naturally remove the dead or damaged skin leaving healthy skin to continue growing. The nibbling is a pleasant experience once the individual becomes used to the initial sensation.There is a symbiotic relationship between human beings and the natural environment in which we live. This fish therapy is a natural example of where a recreational experience provides therapeutic benefits on the skin. In addition to removing dead skin the fish pedicure is also able to remove dirt and provide a deep cleansing treatment to the skin. This in turn also helps new skin regeneration. After the treatment the skin will feel revitalized, smooth and healthy. Many people have reported that their skin has that natural glow once again! The skin has a revived elasticity and that fresh new feel.
What is the Process?
This fish therapy treatment is a very relaxing way to treat the skin. Removing layers of dead skin is part of the natural process of skin growth and this innovative treatment is enjoyable and therapeutic. The sensation of the fish massage stimulates nerve endings which in turn stimulate the nervous system in a similar way to acupuncture treatments which help relieve the body of tiredness and provide a form of relaxation.
Are there any side effects ?
This naturally occurring behaviour has been known to help treat various skin conditions with no side effects. Whilst no one would claim that the therapy is a natural cure to any one skin condition it is certainly a beneficial treatment. An enjoyable, comfortable and wonderful feeling during the nibbling process that can’t be described in words. It will bring no harm because of the tameness and gentleness of the fish.
Dr Fish Spa Therapy Startup Guide at  $49.95 is the resource for you.
What The Book Covers
  • Introduction to Running a Fish Spa
  • Choosing a Premises and Requirements
  • Guide to Buying Fish & Suppliers
  • Guide to Buying Tanks & Suppliers
  • Building Your Own Tanks
  • Tank Setup
  • Tank Maintenance
  • Transporting Fish
  • Introducing Fish
  • Fish Maintenance & Care
  • Emergency Fish Care & Health Problems
  • Customer Service & Treatment Provision
  • Health Questionnaires
  • Computer System, Timers and Till Setup
  • Additional Product Sales & Suggestions
  • Insurance and Due Care

Wednesday, 23 May 2012




What is Marine Fish Oil?
Marine Fish Oil is a highly purified, concentrated fish oil supplement recommended by healthcare professionals to help patients enhance intake of omega-3 fatty acids that are often inadequate in the diet. The specific types of omega-3 fatty acids found in fish, called EPA and DHA, are important to healthy cardiovascular, neurological, and
immune system function. 

How does fish oil benefit heart health?
EPA and DHA in fish oil help maintain normal blood triglyceride levels, reduce platelet stickiness, maintain elasticity of artery walls, stabilize heart rhythm, and assist in regulating blood pressure. They also keep blood from clotting too quickly. Studies show reduced risk of heart attack and stroke. Bypass surgery and angioplasty patients reportedly also benefit from fish oils. Clinical trials have shown that fish oils are safe for heart disease patients. 

How much Marine Fish Oil should should one take?
One to four softgels daily is recommended for general use; however, as many as 10 or 12 softgels may be  recommended by physicians for patients predisposed to neurological conditions such as Alzheimer’s or bipolar disorder, coronary artery disease, diabetes, or rheumatoid arthritis. The amount can be divided and taken several times per day or taken all at once. Marine Fish Oil is safe for adults, children, and nursing mothers

How is the purity of Marine Fish Oil assured?
A rigorous purification process called molecular distillation is used to remove heavy metals such as mercury, as well as PCB’s, pesticides, and other impurities found in fish and crude fish oil products. This advanced technology allows higher amounts of fish oil to be taken safely when medically indicated.

How long will it take to see benefits?
Some patients detect noticeable benefits in one to two weeks. However, it may take several months for full benefits to be seen as each individual is different.  

Fisheries Information

In order to understand the production of marine oils it is important to understand where these products come from. The literature has been full of reports, cartoons and articles detailing the potential collapse of global fisheries and some have even predicted that by 2048 the wild fisheries will be depleted [1]. These predictions have unsettled the markets that depend on marine oils and in some cases have led to panic buying and speculation in these markets which eventually leads to major swings in the prices of these products.
When you look at the historic global landings of fish and crustaceans over the period 1950 - 2008 (the latest year for this data), you find that the global fisheries production seems to be growing at a rate of about 11.5% per year. However closer examination of the data shows that most of the growth from 1992 to the present is due to aquaculture and over that same period growth in the global capture fisheries has been relatively static. Extension of the data indicates that if everything remains the same, the capture and aquaculture lines will cross in 2030 or so. Figure 1 compares ocean and inland capture fisheries to aquaculture production over the period 1990 - 2008 [2].
Figure 1

Aquaculture is an important consideration when discussing marine oils since on the one hand it is the major consumer of fishmeal and oil and on the other could be a potential source of fishmeal and oil, from the by-products, when the fish are processed.

Production of Crude Marine Oils

Raw Material

According to the Food and Agriculture Organization (FAO) of the UN [3], raw material used for the production of fishmeal and fish oil falls into several categories:
  1. Fish caught specifically for reduction to fishmeal and fish oil such as menhaden, anchovy, capelin and sardines.
  2. Incidental or by catch from another fishery, for example according to Alverson the global discards amounted to 27.0 million metric tons (mmt) with a range of from 17.9 to 39.5 mmt with shrimp by catch accounting for 11.3 mmt [4].
  3. Fish by-products from the edible fisheries such as cuttings from filleting operations, fish cannery waste, roe fishery waste and more recently surimi processing waste.
When we talk about the Omega-3 market we could also include krill, squid, seal, marine and freshwater algae, GM yeast and GM oilseeds even though these products are not derived from fish.
The original three categories have similarities; the fish are of little edible use or the raw material is a waste and of no edible value and in fact may present a potential disposal problem and the raw material contains a high percentage of oil and or bones [3]. The latter two categories, by catch and offal produce small volumes of oil compared to the volume produced from whole fish because the traditionally edible species are primarily non-fatty and generally classified as "white fish". However salmon and tuna for example do contain oil in the heads and these are processed to provide fish oil.
The major fisheries involved in the production of marine oils and their sources are shown in Tables 1 and 2.
Table 1. Species of fish caught for fish oil and fishmeal production.
 AnchovyPeru, Chile, So. Africa, Namibia, Mexico, Morocco
 Jack (Horse) MackerelPeru, Chile, China, Vanuatu
 CapelinNorway, Iceland, Russian Federation
 MenhadenUSA: Atlantic and Gulf of Mexico
 Blue WhitingNorway, UK, Russian Federation, Ireland
 Sand eelDenmark, Norway, Faroe Islands
 Norway PoutDenmark, Norway, Faroe Islands
 SpratDenmark, Russian Federation
Source: [2]

Table 2. Fish trimmings and other non-fish species used or could be used for fish oil and fishmeal production.
 Catfish spp.USA, Vietnam
 Tuna spp.Thailand, Japan, USA, Australia, South Korea, China, France, Ecuador, Maldive Islands and many others
 Salmon, farmedNorway, UK, Ireland, Canada, Chile, Faroe Islands, Australia
 Salmon, wildCanada, USA-Alaska, Japan, Russian Federation
 Sardine/PilchardPeru, Chile, South Africa, Namibia, Japan, Spain, Mexico
 White Fish spp.UK, USA-Alaska, Canada, Chile
 DogfishCanada, USA
 PollockUSA-Alaska, Russia
 Atlantic HerringIceland, Norway, Denmark, UK, Faroe Islands, Sweden, Ireland, Canada
 Mackerel spp.UK, Peru, Chile, South Africa, Ireland, Norway, Denmark, Spain, Namibia, Russian Federation, China, Thailand
 Horse MackerelAngola, Mauritania, Morocco, Namibia, South Africa, Turkey, France, Ireland, Latvia, Lithuania, Netherlands, Norway, Russian Federation, Spain, Ukraine, New Zealand
 Hoki (Blue
Australia, New Zealand
Non- fish species
 KrillNorway, Poland, Ukraine, Japan, South Korea
 SquidArgentina, Chile, Peru, USA, Japan, China, South Korea, Russian Federation, France, Portugal, Spain, UK, Morocco, Mexico, Hong Kong, Taiwan, Ghana, Mauritania, South Africa, Senegal, Tunisia, Falkland Islands, Indonesia, Malaysia, Philippines, Thailand, New Zealand
 Single Cell
USA, Japan, Australia, Canada, USA (Hawaii), Israel, India


Fish used for the production of fishmeal and fish oil are often caught by large ocean going vessels working fairly close to the processing plant, but usually not more than 3 days away. Large factory ships tend to stay out for most of the fishing season and transfer product and supplies to carrying vessels which then return to a shore facility. Purse seining is the principal catch method used. Since some species feed and live near the surface (pelagic) they are easily visible from the vessel or from the air. Airplanes are used to spot the schools and direct the fishing vessels to them or they are spotted from the fishing vessel when birds are seen feeding on the schools. When the school is located, the vessel releases two purse boats that have a net stretched between them. The purse boats encircle the school, and then close the net to form a purse or bag. The net is then retrieved to concentrate the catch, after which the mother ship comes along side and pumps the fish and seawater aboard. The water is separated, discharged back overboard and the fish are dropped into refrigerated holds. Other fisheries utilize a single purse boat which acts as an anchor while the mother ship encircles the school of fish.
Other catch methods involve trawls which can be hauled at various depths depending upon the location of the fish, baited long lines, pole line and hook and trap nets. In every case, the fishing method will depend upon the species of fish being sought, its location and depth and the size of the schools.
Once the fish are delivered to the factory, they are unloaded from the vessel by one of several methods. These can be divided into wet and dry methods. Dry methods work best with small catches of edible fish while wet unloading seems to work best for large catches. The downside of wet unloading is that unless the water is processed a large portion of the catch (yield) can be lost. It becomes much more complicated when the unloading water is sea water since processing will result in a high salt content in the fishmeal, which is not desirable [5].

The Wet Reduction Process

The processing techniques involved in commercial production of edible fats and oils vary according to the type of raw material. Fish reduction to produce oil and fishmeal, except for solvent extraction, generally employs the same principles, techniques and equipment common to the production of the other edible fats and oils. In general, fish are processed by the wet reduction method in which the principal operations are cooking, pressing, separation of the oil and water with recovery of oil, and drying of the residual protein material. Continuous processing from the time the fish are landed optimizes efficiency and maximizes product quality [3].
There are a number of processes that can be used to convert raw fish and cuttings into fishmeal and oil. These fall into several categories defined as wet rendering, hydrolysis, silage production (autolysis), dry rendering and solvent extraction. The wet rendering process is used in the majority of the factories that produce fish oil worldwide. This process is universal, i.e. factories all over the world both on land and on ships employ it with slight differences in equipment type, but the major steps of cooking, pressing, separating, and drying are always present. These steps can be seen in Table 3.
Table 3. Processing steps used to produce fish oil from fish and fish cuttings.
 CookingSteam cooking ruptures the fat cells, coagulates the protein and releases the oil
The cooked fish mass is screened to separate free liquid from the solids
 PressingPressing mechanically expresses the free liquid from the solids producing a press liquor (oil and water) and a press cake (semi-moist meat and bones). Some factories have used tricanters instead of presses to separate solids, oil and water.
 Press liquor
This is 3 step process; decanters separate fine solids from the liquid fraction, separators split the liquid fraction into fish oil and water (stickwater), and polishing water washes the crude fish oil before it is pumped to storage.
 EvaporationStickwater contains about 8% solids which are concentrated in multiple effect or waste heat evaporators to about 40-50% solids. If the factory uses steam dryers then the waste heat from the dryer can be used to heat and evaporate the stickwater.
 DryingThe drying process is generally done in 2 stages. The solids from the decanter separation and the press cake are mixed and partially dried. The partially dried fishmeal is then mixed with the concentrated stickwater and the drying is completed to about 10% moisture. Factories use steam and indirect hot air dryers but older factories still use the old direct fired hot air dryers.
 GrindingGrinding reduces the particle size of the fishmeal.
 Cooling and
The fishmeal is cooled and antioxidant is added. Generally ethoxyquin is the antioxidant of choice but for certain markets natural antioxidants based on tocopherols are used.
 PackagingThe fishmeal is packaged in 50 kg bags or 1000 kg totes. The fishmeal can also be stored in bulk piles or in silos.
 Optional Fish Oil
If the crude fish oil is destined for the Omega-3, animal feed, aquaculture or pet food market and if analyses indicate the presence of dioxins, furans and or polyaromatic hydrocarbons (pah), it can be treated with activated carbon to reduce the levels of these compounds.
Source: Bimbo 2000 [5]

A typical flow diagram of the wet reduction process can be seen in Figure 2.
Figure 2b
Figure 2. Wet reduction process to produce crude fish oil

Other Production Methods

In addition to the wet reduction process, which is the primary method for producing crude marine oils, there are other production methods that are being or have been used to produce these oils.

Hydrolysis (Enzymatic)

Hydrolyzed fish proteins are produced by employing proteolytic enzymes either from the fish themselves (autolysis/silage) or from other sources (hydrolysis). The enzymes can be of either animal, vegetable or microbial source and accelerate the breakdown of the proteins into smaller units (peptides). Hydrolysis can also be accomplished chemically under acidic or alkaline conditions. By using some of the newer enzymes available on the market, a process can be developed to recover fish peptides of various lengths with specific functionality [6]. Although the process can be used with any fish, it is primarily used for white fish or offal low in oil. In cases where oily fish are hydrolyzed, the processor must recover the oil phase without denaturing the proteins or face supplying a high fat hydrolyzed protein product or a protein product with reduced functionality. It has been difficult to achieve a commercially viable product from fatty fish that is both functional and low in fat. The hydrolysis process is shown in Figure 3.
Figure 3
Figure 3. Hydrolysis process to produce crude fish oil.

Silage Production (Autolysis)

Silage production is a simplified, low cost, hydrolysis process. Fish silage is liquefied fish stabilized against bacterial decomposition by an acid. The process involves mincing of the fish followed by the addition of an acid for preservation. The enzymes in the fish gut break down the fish proteins into smaller soluble units and acid helps to increase their activity while preventing bacterial spoilage. Formic, propionic, sulfuric and phosphoric acids have been used. Normally, about 3-4% of acid is added so that the pH remains at or below 4.0. Strong mineral acids require neutralization before feeding the final product. Silage might be defined as a crude form of hydrolyzate.
Silage made from white fish offal does not contain much oil, but when made from fatty fish it is necessary to remove the oil. The composition of the silage will be very similar to the starting raw material. Fish silage of the correct acidity is stable at room temperature for at least 2 years without decomposition. The protein becomes more soluble, and the amount of free fatty acids increases in any fish oil present during storage. Silage production offers a solution to the handling of fish waste when the logistics of delivering the waste to a fish reduction plant are not economical. Silage can be produced in large or small containers both on the vessel and on shore. If the silage is processed quickly to recover the oil, it is possible to make an acceptable fish oil product. The silage process is shown in Figure 4; it can be carried out in 2 stages, producing a crude silage with very low capital expense and then producing a concentrated silage and fish oil which requires a high capital expense.
Figure 4
Figure 4. autolysis or silage process to produce crude fish oil

Dry Rendering

The dry rendering process, which is commonly used to prepare animal proteins and fats, is not normally used in the manufacture of fishmeal and oil. However the process is used with catfish by-products. In this process the raw material is "cooked" to remove the water (essentially the drying process in the fishmeal wet rendering process. The resultant dry cake is then pressed to remove any oil. Because the water has been removed, the lipid fraction can contain high levels of phospholipids. The phospholipids normally hydrate in the wet rendering process and are recovered with the water fraction. In the dry rendering process, they are not hydrated and therefore remain dissolved in the lipid or oil fraction. Since there is interest in the fish phospholipids, it is possible to produce a PL fraction by hydrating the oil (also called degumming). The dry rendering process is shown in Figure 5. Further embodiments of this process, which is used in the processing of animal protein by-products have been reviewed by Bimbo [7].
Figure 5
Figure 5. Dry rendering process to produce crude fish oil.

Solvent Extraction

Solvent extraction to produce fish protein concentrate (FPC) is another process that could yield fish oil. FPC can be defined as any stable fish preparation intended for human consumption in which the protein is more concentrated than in the original fish. Water and fat together constitute about 80% of the whole fish, with the fat itself in some species being as high as 20%. The manufacture of FPC involves the removal of most of the water and some or all of the fat. Methods developed so far are based mainly on the use of chemical solvents to remove water, fat and fishy tasting components either from the raw fish or from fishmeal. The solvents most successfully used to make FPC are ethanol, n-hexane, isopropanol, or ethylene dichloride. Normally the solvent is recovered and used over again. The recovered fat is usually mixed in an azeotropic mixture with water, solvent, and water soluble components. Separation of this azeotrope to recover the fat sometimes presents problems [8]. Unfortunately, although FPC was nutritionally acceptable it had very poor functionality and most processes used to produce the product have been abandoned or replaced by processes that produce a functional protein concentrate such as fish protein isolates, and surimi.

Acid-Alkali Aided Process

Kristinsson and Demir [9] described a process that can be used to produce a fish protein isolate and a fish oil by use of alkali and or acid to digest the muscle proteins. Originally described as a replacement for the surimi process it is now being evaluated as a method for recovery of proteins from fish by-products. If the by-products are from oily fish, there would also be a marine oil product produced. The acid/alkali aided process is shown in Figure 6.
Figure 6
Figure 6. Acid/alkali aided process for fish protein isolates and fish oil production.

Other Sources of Marine Oils

In addition to the commodity marine oils there are other marine oils produced for the Omega-3 market.


While currently not a major source of oil for the Omega-3 fatty acid market, krill oil has generated a great deal of interest in the last few years because of the unique form of the lipid in the krill. Krill is a term applied to describe over 80 species of open-ocean crustaceans. Of the seven species of euphausiid crustaceans commonly found in the Southern Ocean, only two of them regularly occur in dense swarms and are of particular interest to commercial fisheries: E. superba and E. crystallorophiasE. superba is the species commonly referred to as “Antarctic krill” and it is a widespread species, which is subject to significant commercial fishing in the Southern Ocean and in the waters around Japan. The total global production amounts to 150,000 – 200,000 metric tons annually and if the krill contained 3% lipid and all of it was recoverable as krill oil, the total production would be about 4500 - 6000 metric tons.
Krill are very delicate and contain powerful enzymes within their digestive tract. This causes the krill to deteriorate rapidly once they are caught so processing must be done at sea to preserve the quality of the raw material. There is very little published on how the krill are processed on-board the ships. There are several possible options that could be employed:
  • Option 1. The krill can be processed on board the vessel producing krill meal and krill oil by the standard fishmeal process. This is shown in Figure 7.
Figure 7
Figure 7. Wet reduction at sea for krill oil production.
  • Option 2. The krill can be processed onboard the factory trawler by simply drying the biomass. This would remove a large part of the weight of the krill, the water, denature the enzymes and essentially stabilize the material for further processing at a land-based facility. The dried krill meal would be high in fat so the fat would be removed at a shore-based facility. This is shown inFigure 8.
Figure 8
Figure 8. Dry biomass at sea and solvent extract at shore facility to produce krill.
  • Option 3. The krill could be immediately frozen into blocks at sea and transported to a shore side facility for processing into krill meal and krill oil using conventional rendering, or solvent extraction. This is shown in Figure 9.
Figure 9
Figure 9. Freeze krill at sea and process frozen blocks at shore facility to produce krill oil.
  • Option 4. The krill could be enzymatically digested on the vessel and the digest separated into a krill oil and krill digest. It would be necessary to concentrate the digest to a paste, which could be done at sea or at a land based facility. The digestion process could be either the standard fish silage process or a more sophisticated enzymatic digestion process. This is shown in Figure 10.
Figure 10
Figure 10. Enzymatic digestion of krill at sea to produce krill oil.
It would not be practical to solvent extract aboard the vessel because of safety concerns, but it might be possible to use a compact supercritical fluid extraction facility on the vessel. There are a number of recent patents dealing with the use of supercritical fluids for the processing of krill oil [10-13].

Fish and Other Livers

Fish liver oils have been used as far back as the Middle Ages and populations in Norway, Iceland, Greenland and Scotland have used them for thousands of years [14]. The most important raw material for the production of liver oils comes from the fisheries for cod, coalfish, and haddock. The livers of other species including several species of shark have also been used in the production of liver oils. In order to obtain high quality, light colored oils with good flavor and odor containing a minimum of free fatty acids, it is important to eviscerate the fish and recover the livers so that they can be processed as quickly as possible.
In recent years, identified cod liver oil has been displaced by liver oils from various other fish species such as Alaska Pollock, other gadoid species and hake liver oils. Generally, steam cookers are used to extract the oil from the livers. Low-pressure steam is piped into a tank containing the livers and the heat cooks the livers. When the steam condenses a layer of hot water is produced which floats the oil. The oil is then separated and pumped into a storage tank. Some liver oils are extracted at sea on board trawlers when they remain at sea for long periods of time.
A process that treats the liver residue with caustic soda was developed in Iceland. After the medicinal grade oil is separated, the residue is then treated with caustic soda. This destroys the protein and the oil floats to the surface and is recovered as veterinary grade cod liver oil. This grade is darker in color and contains a higher level of vitamins than the medicinal grade.
In a more modern operation, the livers are ground and pumped over magnets to remove tramp metal especially hooks which come from the freezing plants. The livers are heated and allowed to stand for a period of time to break down the proteins. The livers are then run through decanters to remove solids, and the liquor is collected in kettles, heated to 95°C and then separated. Modern three way separators are used and the crude cod liver oil is collected and pumped to the refinery. In the refinery the oil is alkali refined to remove free fatty acids, washed and dried in a vacuum tower and then winterized to remove stearines. The result is medicinal grade cod liver oil (Figure 11).
Figure 11
Figure 11. Production of cod liver and other liver oils [22].

Single Cell Oils

Certain single cell organisms, natural and genetically modified, have been shown to be rich sources of the Omega-3 fatty acids EPA (eicosapentaenoic acid or C20:5n-3) and DHA (docosahexaenoic acid or C22:6n-3) or the Omega-6 fatty acid ARA (arachidonic acid (eicosatetraenoic acid) or C20:4n-6). In addition to the products currently on the market, there are a number of cross-over technologies taken from the growing bioenergy industry where organisms grown to produce algal oil for biofuels were found to produce oils relatively rich in the desired Omega-3 fatty acids.
These organisms can be grown in fermentors or in outside ponds (where the climate permits). The advantages and disadvantages of the two methods is not within the scope of this document, however, regardless of the method employed, the subsequent processing would be the same; namely separation of the biomass from the liquor, drying of the biomass, recovery of the microbial or single cell oil and the subsequent refining of the oil. Wynn and Ratledge [15] discussed this process in great detail. The processing steps for making single cell oils are shown in Figure 12.
Figure 12
Figure 12. Production of single cell oils
Several groups have listed a number of potential organisms, and their oil content range, that could be used to produce algal oils for biofuels. Some of these are already being used to produce Omega-3 oils [16-18]. The list is shown in Table 4.
Table 4. Oil content of some micro-organisms
 MicroorganismOil Content
(% dry weight)
 Botryococcus braunii25 - 75
 Chlorella sp.28 - 32
 Crypthecodinium cohinii20
 Cylindrotheca sp.16 - 37
 Dunaliella primolecta23
 Isochrysis sp.25 - 33
 Monallanthus salina>20
 Nannochloropsis sp.31 - 68
 Neochloris oleoabundans35 - 54
 Nitzschia sp.45 - 47
 Phaeodactylum tricornutum20 - 30
 Schizochytrium sp.50 - 77
 Tetraselmis sueica15 - 23
 Ulkenia sp.70 - 75
 Euglena14 - 20
 Yarrowia lipolyticaGM27 - 37
Source: Burns 2010 [16], GOED 2011[17], Ratledge and Cohen 2008[18]

Marine Oil Information

On a global scale fish body oils make up the majority of the marine oils produced with a small amount of oil coming from fish and shark livers, krill, squid, marine mammals, marine and freshwater algae, and yeasts. Six geographic locations account for most of the global production of marine oils. These are shown in Figure 13.
Figure 13
Figure 13. Global marine oil production.
The market for marine oils has been evolving since the early to mid-1990s as the market began moving away from hydrogenation for the margarine and shortening to aquaculture. The Omega-3 market has been slow to develop but is growing and today represents about 10-12% of the total marine oils produced. This can be seen in Figure 14.
Figure 14
Figure 14. Change in the market structure of fish oil.
A more descriptive figure showing this evolution can be seen in Figure 15. Germany, the UK and the Netherlands were always the major importers of marine oils for the hydrogenation industry, while Norway, Chile, Canada and Asia (predominantly China, Japan and Taiwan) can be considered representative of the aquaculture industry. The Omega-3 market is relatively new and seems to be growing at a pace that will exceed the current and almost disappearing use of hydrogenation.
Figure 15
Figure 15. Fish oil consumption in selected markets.

Processing Marine Oils

In general terms, all crude oils and fats contain minor amounts of non triglyceride substances. While some of these are considered beneficial to the stability of the oil, such as tocopherols and astaxanthin (in salmon and krill oils) which protect the oil from oxidation, other impurities are objectionable because they render the oil dark colored, cause it to foam or smoke or are precipitated when the oil is heated in subsequent processing operations. Other impurities reduce acceptability because of the flavors and odors they produce in the fat or because they reduce stability and shelf life of the foods to which the fats are added. Extra virgin olive oil is the exception to the rule.
Some impurities are common to all fats regardless of the source or end use:
  1. Suspended matter (insoluble impurities).
  2. Naturally occurring color bodies.
  3. Free fatty acids.
  4. Volatile, malodorous compounds dissolved in the fat or oil.
These non-triglyceride substances have also been classified according to their effect:
  1. Hydrolytic - moisture, insoluble impurities, free fatty acids, mono and diglycerides, enzymes, and soap.
  2. Oxidative - trace metals, oxidation products, pigments, tocopherols, and phospholipids.
  3. Catalyst poisons - substances which inhibit the hydrogenation reaction e.g. phosphatides, oxidation products, and compounds containing nitrogen, sulfur, and halogens.
  4. Miscellaneous - hydrocarbons, terpenes, resins, sterols, waxes, trace metals and sugars whose effect is less well known but can be classified as contaminants and also may have an effect on the final flavor of the oil [20].
The general objective of processing fats and oils is the removal of impurities which cause the original product to have an unattractive color or taste or which causes harmful metabolic effects. Fats and oils intended for edible purposes are therefore further processed to remove these substances while retaining their desirable features. With the current popular emphasis on "natural" products, certain compounds in fish oils and other oils, which were once undesirable, are now considered desirable and often command a premium price. For example, the dark red color that appears in some fish oils at different times of the year is now considered necessary as a source of pink pigment for farmed salmon. This compound is the carotenoid astaxanthin and it is one of the major selling points for krill and virgin salmon oils in the health food supplement market. Once considered a negative property of fish oil because they impart fishy flavors to red meat animals, cause white paints to yellow and increase the cost of the hydrogenation process, the Omega-3 fatty acids in marine oils are now considered the major positive property of these oils to the point where even oil seed crops are being genetically modified to produce these fatty acids. The evolution of this market for Omega-3 fatty acids has led to a number of new processes for treating marine oils. Such processes are designed to preserve the Omega-3 fatty acids while reducing flavor, cholesterol and other impurities. In some cases these processes were initially very expensive but as the market has evolved, they are now mainstream processes.
The processing steps and the compounds removed by them can be summed up in the Table 5.
Table 5. Processing steps used to purify marine oils
Removal of dioxins, furans, and polyaromatic hydrocarbons (PAH). This can be performed on the starting crude oil if the oil is to be sold into the non-industrial market.
 Oil StorageInsoluble impurities, trace moisture and some phospholipids will precipitate out in the tanks. The combination is known as "foots".
 DegummingPhospholipids, sugars, resins, proteinaceous compounds, trace metals and other materials.
 Alkali RefiningFree fatty acids, pigments, phospholipids, oil insoluble material, water soluble material, trace metals
 Water Washing/
  Silica Treatment
Soaps, oxidation products and trace metals
 Adsorptive Bleaching &
  Carbon Treatment
Pigments, oxidation products, trace metals, sulfur compounds, dioxins, furans, PAH and possibly some pcb's.
 WinterizationHigher melting triglycerides, waxes. Used to enhance the unsaturated triglycerides
 DeodorizationFree fatty acids, mono-diglycerides, aldehydes, ketones, chlorinated hydrocarbons and pigment decomposition products. This is usually the finishing step and results in a bland tasting oil.
 Vacuum Stripping or
  Thin Film, Molecular
   or Short Path
Removal of chlorinated hydrocarbons, fatty acids, oxidation products, PCB and free cholesterol. Sometimes this step is used as a replacement for the deodorization step.

A flow diagram of these processes is shown in Figure 16.
Figure 16
Figure 16. Production of edible and pharmaceutical grade fish oils and derivatives [21]
There are additional processing steps that can be used to convert the refined marine oils to concentrates and relatively purified esters or fatty acids. The processing steps are designed to separate the fatty acids from the glycerine backbone of the triglyceride and then to fractionate the fatty acids or esters into concentrates or relatively pure individual fatty acids. The processing steps are shown in Table 6.
Table 6. Additional processing steps for the production and purification of marine oil omega-3 fractions.
 InteresterificationRearrangement of triglycerides to a more random distribution
 Hydrolysis or
Splitting triglycerides, producing fatty acids or esters with glycerin as a by-product
 Urea ComplexingBy dissolving the fatty acids or esters in ethanol or methanol, adding urea and reducing the temperature, it is possible to precipitate a urea complex which traps the saturates and monounsaturates resulting in a concentration of the polyunsaturates. Depending on the starting oil, the losses can be quite high.
This step can be used to remove free cholesterol or further concentrate the esters or fatty acids.
 Supercritical Fluid
   Extraction (SCF)
Purification of esters or fatty acids to produce 85%+ pure compounds and to remove cholesterol
 Preparative High-
  Performance Liquid
Purification of esters or fatty acids to produce 95%+ pure compounds
 Re-esterificationConversion of concentrated fatty acids or ethyl esters back to the triglyceride form which is considered more natural
Source: Bimbo 1998 [21]
Many of the processes described above can be modified or in some cases improved by the use of enzymes. Some of these enzymatic processes have been reviewed by others [23].
Figure 17
Figure 17. Production of pharmaceutical and food grade omega-3 marine oils and their concentrates [21]
There are a series of processing steps designed to remove impurities, contaminants, saturated and monounsaturated fatty acids and essentially isolate and recover the Omega-3 fatty acids. The processing steps can be designed around what your end point might be and what your starting raw material is; for example relatively purified fatty acids or esters, concentrates high in DHA or high in EPA [24].